| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228397 |
| Chromosome: | chromosome 9 |
| Location: | 5040573 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398252 | (1 of 13) 1.1.1.300 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCGGCAAGATTGACTTTGACGACCTCA |
| Internal bar code: | CAACAGGTGACGGGCGGGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 411 |
| LEAP-Seq percent confirming: | 97.235 |
| LEAP-Seq n confirming: | 211 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGGGTGGGAGGTATGTG |
| Suggested primer 2: | TTCGGATGTGTGTGTGTGTG |