| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228411 |
| Chromosome: | chromosome 3 |
| Location: | 5511538 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185850 | CPK2 | (1 of 1) PTHR10584//PTHR10584:SF162 - SUGAR KINASE // SUBFAMILY NOT NAMED; putative carbohydrate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGTGTGTTGGAGTGACGCATCGGAAGAC |
| Internal bar code: | GGAGAATAGGTCGCAAACCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 87.3533 |
| LEAP-Seq n confirming: | 2010 |
| LEAP-Seq n nonconfirming: | 291 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCCTTACCCCGACTTTG |
| Suggested primer 2: | AGAGCCTGACCTGTCCTTGA |