| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228411 |
| Chromosome: | chromosome 12 |
| Location: | 178369 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484750 | CLH2 | Chlorophyllase I; (1 of 3) 3.1.1.14 - Chlorophyllase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACTGCGCGCCCCGGGAGGTGAGTGCGGG |
| Internal bar code: | GTTCGCATATCTCTGATACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 21 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATCCACCACAGTATGAC |
| Suggested primer 2: | AGCGTGTGGACACTAATCCC |