Insertion junction: LMJ.RY0402.228418_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.228418
Chromosome:chromosome 11
Location:1039188
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467670 GT90F44,GT90-44 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 44 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACATTCACAGGGCAGATTGTCGTGCAGTG
Internal bar code:ACCCGTAGATGATAAGATGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:99.3827
LEAP-Seq n confirming:322
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTTACGGCCGTCTCTCAACT
Suggested primer 2:GTAGGAAAAGTCCATGCCGA