| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228420 |
| Chromosome: | chromosome 2 |
| Location: | 6100278 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113200 | GLN1,NSG18 | (1 of 2) PTHR20852:SF50 - GLUTAMINE SYNTHETASE CYTOSOLIC ISOZYME 1-1-RELATED; Glutamine synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGGTGCGCCAGCTGGTCACACATCAAC |
| Internal bar code: | AATTTTACATTTTGGTGTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 99.5797 |
| LEAP-Seq n confirming: | 2369 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTCGGGGATTTCTTCT |
| Suggested primer 2: | TGCTCACGCATGTAGACACA |