| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228430 |
| Chromosome: | chromosome 3 |
| Location: | 7247927 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207550 | ADH8,CAD2 | Cinnamyl-alcohol dehydrogenase; (1 of 2) 1.1.1.195 - Cinnamyl-alcohol dehydrogenase / CAD | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGGCGCGCCTAAATGGGAAGACGTGAC |
| Internal bar code: | TCCCATAGCGAATTGTCAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 918 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 521 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCTAGCGATGTAGAGGG |
| Suggested primer 2: | GTGGGGAGAGGGTACGGTAT |