Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.228437 |
Chromosome: | chromosome_13 |
Location: | 3741456 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589450 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGAGGTTGGCGACTGGCTCTCCAAGCCCT |
Internal bar code: | CCTGGATTCGCATTGGCCAATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 591 |
LEAP-Seq percent confirming: | 99.4069 |
LEAP-Seq n confirming: | 7375 |
LEAP-Seq n nonconfirming: | 44 |
LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACATGCCTGGTTGTAAGCC |
Suggested primer 2: | GAGGTAGCTGAACTCCACCG |