| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228439 |
| Chromosome: | chromosome 2 |
| Location: | 1985197 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g088250 | MCT2 | Malonyl-CoA:acyl-carrier-protein transacylase; (1 of 2) K00645 - [acyl-carrier-protein] S-malonyltransferase [EC:2.3.1.39] (fabD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCATCCAGTTGCCCCGGGCCCGGCACA |
| Internal bar code: | TGGTCGCGCCGACGCCGCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 3.59712 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 134 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTCAGTAGGTTCTGTGC |
| Suggested primer 2: | TGGCAGACAGAGGAGATGTG |