| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228448 |
| Chromosome: | chromosome 12 |
| Location: | 3663591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514300 | (1 of 5) 2.3.1.24 - Sphingosine N-acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCATGTCGGTCATGTGGCAGGCGCTGTT |
| Internal bar code: | TCTCTGCGGAGCAATTCGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1023 |
| LEAP-Seq percent confirming: | 99.2033 |
| LEAP-Seq n confirming: | 2615 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGGACGTACACGAAGA |
| Suggested primer 2: | TGGTCCTGTCCTTTGCCTAC |