| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228453 |
| Chromosome: | chromosome_8 |
| Location: | 1034380 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362050 | FAP49 | Flagellar Associated Protein, membrane-associated | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGTCGCGCCATGTGGGCTCGGGACCAAGA |
| Internal bar code: | GACTCTCAGCCTCAAGTGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 94.1437 |
| LEAP-Seq n confirming: | 1061 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAAGGCAGCAAGTAAGG |
| Suggested primer 2: | CGGATCCAGCCCTATCACTA |