| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228465 |
| Chromosome: | chromosome 9 |
| Location: | 1467458 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398200 | (1 of 1) PTHR23244//PTHR23244:SF304 - KELCH REPEAT DOMAIN // KELCH DOMAIN-CONTAINING PROTEIN 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGAGCCAGAGAAGGGGCGGAGGCGGT |
| Internal bar code: | GACGGTCAGATCGCCTGTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.7035 |
| LEAP-Seq n confirming: | 10088 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACATGTTTCATTCCGCAC |
| Suggested primer 2: | GGACAATGAGGCCAAGGTAA |