| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228475 |
| Chromosome: | chromosome 12 |
| Location: | 8356220 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549300 | MIP1 | (1 of 2) PTHR19139:SF180 - AQUAPORIN-1; Aquaporin major intrinsic protein 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCTGTATGGCAACGTGTGGGGGGGGGAG |
| Internal bar code: | TATCGTGGCCTCCGGGCAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.6772 |
| LEAP-Seq n confirming: | 3397 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAGCCAGCTTCGACAACA |
| Suggested primer 2: | AGCTATTGAGGTTAGGCGCA |