Insertion junction: LMJ.RY0402.228488_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.228488
Chromosome:chromosome 7
Location:1719392
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g325711 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGGGCACTTCTTGGCCAGCCGCTCATT
Internal bar code:CGATTCGACAAAATCTGGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1304
LEAP-Seq percent confirming:98.628
LEAP-Seq n confirming:5751
LEAP-Seq n nonconfirming:80
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCTACAGTCTCCCAGCA
Suggested primer 2:AACCTTTGGAATGAAACCCC