| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228497 |
| Chromosome: | chromosome 8 |
| Location: | 2498281 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g371957 | RAPTOR1,RAPTOR | Regulatory-associated protein of TOR; (1 of 1) K07204 - regulatory associated protein of mTOR (RAPTOR) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCCGCCGGCGGCGGCAGTGTGGGCGGC |
| Internal bar code: | TTGTGTCAGCCCCCTCTTTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGGAGAAGTACTCACAG |
| Suggested primer 2: | TGGCTACAACTTCGCTCCTT |