| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228499 |
| Chromosome: | chromosome 7 |
| Location: | 325368 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314450 | SMM22,CGL33B | (1 of 1) PTHR12176:SF19 - S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN; conserved methyltransferase of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAACTACCGTGTGCAGGCACGGGAAGG |
| Internal bar code: | CTTTGGCGCGGGTCATGAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 99.3025 |
| LEAP-Seq n confirming: | 2705 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACGGACGGTACATGCAG |
| Suggested primer 2: | CCCAGAACTAGCTGCCAAAG |