| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228499 |
| Chromosome: | chromosome 7 |
| Location: | 325405 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314450 | SMM22,CGL33B | (1 of 1) PTHR12176:SF19 - S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN; conserved methyltransferase of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGACCCACATGCTTGTGCAACAAACCTG |
| Internal bar code: | TTAGTTGTATTATTATTGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 99.5169 |
| LEAP-Seq n confirming: | 9064 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACGGACGGTACATGCAG |
| Suggested primer 2: | CCCAGAACTAGCTGCCAAAG |