| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228524 |
| Chromosome: | chromosome 1 |
| Location: | 5759759 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041100 | HLM3 | (1 of 1) PTHR12197:SF75 - HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2; Histone-lysine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCATCCAGCAATCCCATCAGCGGTCAC |
| Internal bar code: | CAATGGCATCGGGTTTCGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 85.005 |
| LEAP-Seq n confirming: | 856 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCATGCTAGCTCCAAGT |
| Suggested primer 2: | GTGTAAGTGCGAGCGGTGTA |