| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228525 |
| Chromosome: | chromosome 10 |
| Location: | 6042676 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463150 | DCI1 | Enoyl-CoA hydratase/isomerase family; (1 of 1) PTHR11941//PTHR11941:SF43 - ENOYL-COA HYDRATASE-RELATED // DELTA(3,5)-DELTA(2,4)-DIENOYL-COA ISOMERASE, MITOCHONDRIAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAGAGCGCCTCGTGAAAGCAGTTATGC |
| Internal bar code: | GAGTGTCATAACGGTAGACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1009 |
| LEAP-Seq percent confirming: | 99.5084 |
| LEAP-Seq n confirming: | 3036 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGACCAGGCATTTCACT |
| Suggested primer 2: | ATGTCTCGGGCAGTTCGTAG |