| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228528 |
| Chromosome: | chromosome 10 |
| Location: | 597459 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421800 | (1 of 21) PF02469 - Fasciclin domain (Fasciclin) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTGTCAAAGTTGCTCCGTGCACGCCC |
| Internal bar code: | GGTTAGCGGGGGTTGGCTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.5327 |
| LEAP-Seq n confirming: | 426 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGCGAAAGACAAGGTGG |
| Suggested primer 2: | CACTCCTGGAGGCTTAGCAC |