| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228528 |
| Chromosome: | chromosome 10 |
| Location: | 1621827 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429650 | (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTAAGACTGGTGGCGGTCTGCAGATGCG |
| Internal bar code: | CTCTTACTGATTCCGTTGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 90 |
| LEAP-Seq percent confirming: | 99.8512 |
| LEAP-Seq n confirming: | 671 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACAGGGTTTCAAACGAC |
| Suggested primer 2: | CGAGTGCAAGTTCGACGATA |