| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228530 |
| Chromosome: | chromosome 13 |
| Location: | 1432835 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTGGCTGACTGCGTAGGCTCCCTGTTG |
| Internal bar code: | GAGCCCGGTATGTCGCAGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 97.032 |
| LEAP-Seq n confirming: | 3400 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGGAGAGGGAGGTGT |
| Suggested primer 2: | CTTCCAGCCAGCAACTTTTC |