| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228534 |
| Chromosome: | chromosome_6 |
| Location: | 3827876 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278170 | FAP237,FAS7 | Fasciclin-like protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTTCTTCGCGCCGACCAACGAGTGAGTGG |
| Internal bar code: | TCTGCTTAACCGGTGTAGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1219 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACATGTAAGGTGGGCAGG |
| Suggested primer 2: | TGTCACCGTCACCATCAACT |