| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228540 |
| Chromosome: | chromosome 5 |
| Location: | 2497231 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCATGGAAGCCACCACGTCGCATGGTCG |
| Internal bar code: | ATGATGGACCAACACATTAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1023 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCATTGCTGAGGACACGA |
| Suggested primer 2: | GTACTCTGTCGCAGCTTCCC |