| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228540 |
| Chromosome: | chromosome 9 |
| Location: | 5267952 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399663 | FXL7,FXL4 | FixL-like PAS domain protein; (1 of 11) PF13426 - PAS domain (PAS_9) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTCGGCAAGAGCATACGGTGCCGGGAC |
| Internal bar code: | GACGCCCGCGGCGTTGTATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 99.3827 |
| LEAP-Seq n confirming: | 3059 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCATGTGCGTTAGGGAGG |
| Suggested primer 2: | CCATCACGTACATCACCTGC |