| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228546 |
| Chromosome: | chromosome 3 |
| Location: | 6623609 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196700 | (1 of 4) PF01679 - Proteolipid membrane potential modulator (Pmp3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACATATGGCGTGATCCGCGAGCAATGTG |
| Internal bar code: | CTCAAGGTGACTTCTTCAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 99.3 |
| LEAP-Seq n confirming: | 6100 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACAGTGCGACACGTTAC |
| Suggested primer 2: | GCACAGAAGTGCATTGGCTA |