| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228579 |
| Chromosome: | chromosome 7 |
| Location: | 4131159 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342750 | (1 of 1) K18106 - D-galacturonate reductase (GAAA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACAAGCAGTTGTGTGTGTGTGTGTGTGT |
| Internal bar code: | CGTATAGTGGGTGCTTCCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 85.3295 |
| LEAP-Seq n confirming: | 2059 |
| LEAP-Seq n nonconfirming: | 354 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATGCTTACCACACACTG |
| Suggested primer 2: | TGTCTACCTCACCACCACCA |