| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228581 |
| Chromosome: | chromosome 4 |
| Location: | 3114353 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225800 | CPLD71 | dihydrolipoyllysine-residue acetyltransferase; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGATGAGGGGACGCTAGGGTTGCCAGC |
| Internal bar code: | AGTGTTTGGTTCTGATGAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 97.2498 |
| LEAP-Seq n confirming: | 1273 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGAAGAACGGTGGTGAGG |
| Suggested primer 2: | CTGCTATGAGAATGCGTCCA |