| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228588 |
| Chromosome: | chromosome_9 |
| Location: | 3668865 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390615 | FAP12 | Triacylglycerol lipase and Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTCTGTGGTGGGAGCAAGAGAAGAAAGG |
| Internal bar code: | GTCGGTTGCGGCATCGACAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 98.9734 |
| LEAP-Seq n confirming: | 3085 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAATGGATGGAGGAGGAG |
| Suggested primer 2: | CACAGAGTCGCCACGAGTTA |