| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228595 |
| Chromosome: | chromosome 16 |
| Location: | 1712700 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654800 | CPC2 | Central pair microtubule complex 2; (1 of 1) PTHR24198//PTHR24198:SF25 - ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCAGCTGCAGCCGCGCGCCGTGCTCCA |
| Internal bar code: | GAACGCCATGCGAGAGACAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 91.4494 |
| LEAP-Seq n confirming: | 877 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTTTGAGGCTGCTGTTT |
| Suggested primer 2: | CCTGTTGCTGCTTCGTGATA |