| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228603 |
| Chromosome: | chromosome 9 |
| Location: | 4882816 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397771 | OPR117 | OctotricoPeptide Repeat protein 117 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAACCGCAACTGAGCGAGAGGGCCATG |
| Internal bar code: | TCAAGCTTGCCCTCTCGATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 98.1308 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGGAAACTGCAGACGAA |
| Suggested primer 2: | CCAGTGGTGGTTTCGTCTCT |