| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228620 |
| Chromosome: | chromosome 14 |
| Location: | 1862764 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620500 | (1 of 6) IPR001471//IPR016177 - AP2/ERF domain // DNA-binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCAGCCACACATTGGATTCGCCTTAAT |
| Internal bar code: | AATCAACTTGGAAAAGTTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 99.8195 |
| LEAP-Seq n confirming: | 553 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCAAAGTGATCTGGGTC |
| Suggested primer 2: | TACACCTGCCACCGACATAA |