| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228630 |
| Chromosome: | chromosome 6 |
| Location: | 5398704 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283900 | (1 of 1) PTHR16007//PTHR16007:SF15 - EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED // TRANSMEMBRANE PROTEIN 45B | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGTCGGCGGGGAGAAACACTGCCAGA |
| Internal bar code: | TGCCTCACTTCAAGCGACTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1219 |
| LEAP-Seq percent confirming: | 99.4894 |
| LEAP-Seq n confirming: | 8769 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTTAGCAGGCGAATAGG |
| Suggested primer 2: | GCTTGATGTGAAAAGCACGA |