Insertion junction: LMJ.RY0402.228644_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.228644
Chromosome:chromosome 2
Location:6882861
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTTGCGGCTGAGCGCCGCTATGCATGCGG
Internal bar code:GGTTCTCAGGCTGTCGTTAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:250
LEAP-Seq percent confirming:97.1903
LEAP-Seq n confirming:761
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GACTAAGAGCTGGTCGACGG
Suggested primer 2:TGCTCTTGCCTTCTCCATTT