Insertion junction: LMJ.RY0402.228644_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.228644
Chromosome:chromosome 7
Location:5412284
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g350300 (1 of 31) IPR016187 - C-type lectin fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTGCTGCATCCACCACCTCAAACACGCA
Internal bar code:TAGCCTATCGCTATTATATTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1034
LEAP-Seq percent confirming:99.0085
LEAP-Seq n confirming:8488
LEAP-Seq n nonconfirming:85
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CATACGTGTACGGGATGCAG
Suggested primer 2:CACACAAACACAGACTGCCC