| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228655 |
| Chromosome: | chromosome 1 |
| Location: | 1687046 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008900 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGGCATGTTGGGGTGCCAGGAGGGCAA |
| Internal bar code: | CGGCCGGTGTCTGGGATTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 96.5659 |
| LEAP-Seq n confirming: | 703 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGGGAAGTGTTGTGGA |
| Suggested primer 2: | GCTCATGGTAGAGGAGCTGG |