| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228656 |
| Chromosome: | chromosome 13 |
| Location: | 2185622 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578201 | RRM13 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCGGCCGGTCGCGCGGCGCAGCCGCAG |
| Internal bar code: | AAGTGGCATGATCATGGCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 99.5016 |
| LEAP-Seq n confirming: | 13975 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGACGCCCTAACCGCTA |
| Suggested primer 2: | CGGTTGAAGAAGAGGCAGAG |