| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228665 |
| Chromosome: | chromosome 6 |
| Location: | 6862096 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCACGGTATGGTGTGGGGATAGGAGGTA |
| Internal bar code: | ACACTCGCCCATTACCTGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 98.8035 |
| LEAP-Seq n confirming: | 12882 |
| LEAP-Seq n nonconfirming: | 156 |
| LEAP-Seq n unique pos: | 148 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTCGGGGTGTATACGTG |
| Suggested primer 2: | GTGAGAAGCGGACGAGAAAC |