| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228674 |
| Chromosome: | chromosome_15 |
| Location: | 153890 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre15.g635000 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATGGGGGGCTGTCGGCAGGGGCGGGGGCA |
| Internal bar code: | GTACGCCTGCTGGGGACCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 99.118 |
| LEAP-Seq n confirming: | 899 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCAGTCCCACACAGGT |
| Suggested primer 2: | TACACACACGCACACACACC |