| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228689 |
| Chromosome: | chromosome_5 |
| Location: | 1174294 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g245550 | PIK1 | Phosphatidylinositol 4-kinase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGACCTGCTTCTGTAGATGTATAGTCGTA |
| Internal bar code: | TGCCCTGGGAACGGTGCAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 99.3173 |
| LEAP-Seq n confirming: | 4655 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGTCTTTTGATTAGGC |
| Suggested primer 2: | TAAATTGTGAATGCCAGCCA |