| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228695 |
| Chromosome: | chromosome 6 |
| Location: | 4162489 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278229 | (1 of 3) IPR000104//IPR004333 - Antifreeze protein, type I // Transcription factor, SBP-box | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGTGATAGGCAGCGTGCTAGTCAGAGT |
| Internal bar code: | GCCCTCTTCAACTGGAATGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1099 |
| LEAP-Seq percent confirming: | 95.8195 |
| LEAP-Seq n confirming: | 1444 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCTTGGAACGCACATAG |
| Suggested primer 2: | GACGAGGGCGAGAAGTACAG |