| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228698 |
| Chromosome: | chromosome 10 |
| Location: | 4639570 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453050 | CYCM1 | (1 of 2) PTHR10026:SF51 - CYCLIN-K; Chlamydomonadale-specific cyclin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTTTACAGAGCGCGCATGTAAGTTGTT |
| Internal bar code: | CGGGGTCAGAGTTCTTCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.2366 |
| LEAP-Seq n confirming: | 1430 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCATAAGTGCAGACGCAG |
| Suggested primer 2: | GCAACCCGCAAAAATCTAAA |