| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228699 |
| Chromosome: | chromosome 10 |
| Location: | 5905370 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462050 | PTK20 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCATGAGGCAGCAGCGCGCCCTGCTTAC |
| Internal bar code: | CATAAAGATAGGGGAGGATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 99.5379 |
| LEAP-Seq n confirming: | 1077 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCCCTAGGCGTATCTC |
| Suggested primer 2: | CTGTGTGGGGTGCTTGTATG |