| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228735 |
| Chromosome: | chromosome 15 |
| Location: | 978823 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639100 | FAP195 | Flagellar Associated Protein 195 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCATAAGCAGCGGGACGAAAAGCAGAAG |
| Internal bar code: | TAGGATATATAGGTTATCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 99.6904 |
| LEAP-Seq n confirming: | 322 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCCCAAACTAAACCG |
| Suggested primer 2: | CCCTGCCTACTTCGCTACAC |