| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228751 |
| Chromosome: | chromosome 4 |
| Location: | 1266476 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215000 | BKT,CBK1,BKT1 | Beta-carotene ketolase; (1 of 1) IPR000104//IPR005804 - Antifreeze protein, type I // Fatty acid desaturase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTTGGGGTGTGCGTTTGTGAGCGCGCA |
| Internal bar code: | AGCGCCGATCCGATTGGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 19.5603 |
| LEAP-Seq n confirming: | 4609 |
| LEAP-Seq n nonconfirming: | 18954 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGCAGGTCGAAGTGGTA |
| Suggested primer 2: | GGAGTCAGAGGCACGAAGAC |