Insertion junction: LMJ.RY0402.228773_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.228773
Chromosome:chromosome 12
Location:7306944
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g557500 (1 of 1) IPR000104//IPR002073//IPR003607//IPR006189//IPR023088 - Antifreeze protein, type I // 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain // HD/PDEase domain // CHASE domain // 3'5'-cyclic nucleotide phosphodiesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGCTACATGCAGCTTCCCGCGTCAGTC
Internal bar code:TGAAGGACCTTGTCGTGAGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:214
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:164
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATAATTCGCCGAAATTGACG
Suggested primer 2:TCACCGCGCATGTAAATAAA