| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228777 |
| Chromosome: | chromosome 16 |
| Location: | 6284937 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674650 | (1 of 1) 3.5.1.59 - N-carbamoylsarcosine amidase / N-carbamoylsarcosine amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAGCATTTCAACGTCGAGAATATCAGC |
| Internal bar code: | GCCGCTGCCGGCGCGTCGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 80.531 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCATGTACATCCCCCAA |
| Suggested primer 2: | GGAACAGTAGCAGCCTCTGG |