| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228791 |
| Chromosome: | chromosome 9 |
| Location: | 6776839 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409350 | RLI1 | (1 of 1) K06174 - ATP-binding cassette, sub-family E, member 1 (ABCE1); Fe-S ABC transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTCACGGGCCGGCGGGATTATCTCCCG |
| Internal bar code: | ATGTCGGACTGAAGCGCCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 99.463 |
| LEAP-Seq n confirming: | 18153 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGTGAGGAAAAGTGAGG |
| Suggested primer 2: | CACGATCACATCACGTCACA |