Insertion junction: LMJ.RY0402.228804_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.228804
Chromosome:chromosome 8
Location:1075332
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g362150 FAP361,FAP154 Flagellar Associated Protein 361 with PAS Sensory Domain; (1 of 28) PTHR31600//PTHR31600:SF2 - FAMILY NOT NAMED // TINY MACROCYSTS PROTEIN B-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCTGGGTCGATCTGACAGTGATTTTGGG
Internal bar code:CAGGCTGACTACACTAGGGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:423
LEAP-Seq percent confirming:78.1809
LEAP-Seq n confirming:1530
LEAP-Seq n nonconfirming:427
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCCTATCTAGCAGTCGC
Suggested primer 2:GTGTTTGTGTTTGGCCTCCT