| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228815 |
| Chromosome: | chromosome_6 |
| Location: | 7273353 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g298500 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATACGGGCCAGGATAGGCACGACCAGGCAG |
| Internal bar code: | CGTGCCTATGGTATCTATTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 99.3517 |
| LEAP-Seq n confirming: | 7050 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGCGATTACTGAAAACA |
| Suggested primer 2: | TGAACACTGCCATAGCGAAG |