| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228825 |
| Chromosome: | chromosome_3 |
| Location: | 6483213 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g194900 | CYA4 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGAGCAAGGATGAGGTGGATATTTCTCGG |
| Internal bar code: | GTCAGGCATCACGGTTGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 98.327 |
| LEAP-Seq n confirming: | 2586 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAAGGCATAGCTGGCTC |
| Suggested primer 2: | CCTAATCTCCTGCAAGACGC |